SCA® Morphology is a module that permits the automatic morphometry analysis and morphology classification of stained sperm samples of mouse and rat observed in brightfield microscopy.
| This analysis module is also available for Human and Veterinary. |
Ideal for testing various drugs, chemicals and radiations for their toxicity. This system adheres to 21 CFR11 guidelines.
More information:
SCA® Morphology last news
SCA® Morphology last news
Analysis improved
Morphology analysis and sperm detection improved.
Morphometric values
Provides the average and deviation of all the parameters analysed.
Export images
Export .bmp or .jpg images including or excluding the analysis.
Individual parameters
Export to Excel or save into the database the individual sperm parameters.
SCA® Morphology main features
SCA® Morphology main features
SCA® detects and analyses the spermatozoa automatically.
Simultaneously evaluates the dimensions of the head and midpiece as well as arc length and chord.
Spermatozoa are recorded as normal or abnormal, following established criteria: Standard, Adaptative or Customized.
Optionally, analysis with motorized stage and SCA® Stage controller is available, improving the capture velocity and making all the process automatic.
SpermBlue® staining appears to be the only available dye for correct visualization and it has been widely validated with SCA®.
SCA® Morphology is an effective and user-friendly and tool for research, allowing the implementation of new terminology and estimations in sperm morphology.
Additional information
Additional information
| Vet Edition |
Tox Edition |
Research Edition |
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| Creating new analysis configurations |  |
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| Modification of analysis box size | |
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| B/W or color capture, Scale, image capture options | |
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| Motorized stage | |
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| Standard animal and User defined. | |
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| Automatic classification for human, swine, bull, ovine, dog, stallion, fish. Rat and Mouse. Morphometry analysis for all species | – | – | |
| Automatic classification for swine, bull, ovine, dog, stallion. Morphometry analysis for all oval head animal species | |
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| Automatic classification for rat and mouse | |
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| Automatic classification for human | – | – | |
| All staining options: Diff quik, Sperm Stain, SpermBlue®, Hemacolor, Papanicolau, Shorr, Cell-Vu Prestained Slides. | – | – | |
| SpermBlue® only | |
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| Particle area modification | |
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| Modification of the Morphology classification / Morphometric values | |
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| Species available: Human | – | – | |
| Species available: All (human, and all animals, including rat and mouse) | – | – | |
| Species available: All animals with oval sperm head (boar, bull, stallion, etc) | |
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| Species available: rat and mouse | – | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Specific digital Basler camera with 780×580 resolution | |
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| Specific digital Basler camera with 1280×1024 resolution | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Total and percentage normal/abnormal sperm analysed | |
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| Basic classification: normal and head, midpiece and tail defects, cytoplasmic droplets | |
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| TZI, MIA, SDI, ERC Index | |
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| Total analyzed and percentage of the spermatozoa with the next morphologic values (*): normal size, acrosome and midpiece; micro, macro, normal shape, tapered, thin, round, pyriform, amorphous, abnormal midpiece in size, angle, insertion, normal and abnormal tail, short, irregular, rolled, multiple, without, angulation. * Morphologic values might change depending on the classification selected. |
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| Morphometric values: head length, width,area, perimeter, ellipticity, elongation, surface roughness, regularity | |
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| Morphometry analysis (average and standard deviation) for rat and mouse.Head: Arc length, Chord length, area, perimeter, linearity, rugosity, regularity, grey level Midpiece: width, area, insertion distance, angle Percentage of each morphological type (It is possible to modify the description) Head: Micro, macro, amorphous Midpiede: Width, asymmetric, angulated Tail: Absent, bent, coiled |
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| Captured images and distribution charts | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| SCA® dataShare | |
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| SCA® Stage Controller |
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| SCA® Capture | |
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| SCA® Viewer | |
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| SCA® Editor | |
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| Analysis under brightfield microscopy, transmitting light to view a specimen that is stained. |
| SCA Morphology analyzes the head, acrosome and midpiece form, shape and size. |
| Tails can be analyzed automatically with the use of the specific high resolution digital camera 1280×1024 or manually if desired. |
| Arc length and Chord are evaluated for rat and mouse spermatozoa. |
| There are several Compatible staining methods depending on the specimen: Diff quik, Papanicoulau, Sperm Blue®, Sperm Stain, Shorr and CellVu prestained slides for human. Sperm Blue® for all animal species, which allows a complete analysis, including acrosome visualization. For each of the staining used the software has corrected factors. |
| Allows the creation of User defined matrix for the classification into normal or abnormal sperm |
| Animal species stained with Sperm Blue® automatic classification criteria: boar, bull, ram, dog and Stallion. |
| Additional descriptions of the Morphologic values can be created |
| Modifiable parameters are: Size of the capture box, staining methods and Classification criteria |
| SCA® permits to capture and analyse up to 500 spermatozoa per sample |
| Head length, width, area, perimeter, ellipticity, elongation, roughness, regularity |
| Acrosome, percentage of head size |
| Midpiece width, area, insertion angle |
| Tail length |
| Arc length and Chord in rat and mouse. |
| Calculation of the following basic parameters: Total analyzed and Percentage of normal and abnormal spermatozoa |
| Total analyzed and Percentage of normal spermatozoa; with head, midpiece and tail defects; and cytoplasmic droplets. |
| Total and percentage of counted spermatozoa having Just one defect, in the Head, Midpiece or Tail. |
| Total and percentage of counted spermatozoa having Multiple defects combined as: Head+ Midpiece, Head+Tail, Midpiece+Tail, Head+Midpiece+Tail |
| Morphologic values*: Total and percentage of analysed spermatozoa having Normal size, Micro, Macro, Normal shape, Tapered, Thin, Round, Pyriform, Amorphous; Normal and Abnormal Acrosome; Normal midpiece, Abnormal midpiece size, angle and insertion; Normal tail, abnormal, short, Irregular, Rolled, Multiple, Without, Angulation |
| Average and deviation of these morphometric values: Head lengh, width, area, perimeter, ellipticity, elongation, roughness, regularity; Midpiece width, area and angle; Acrosome |
| Teratozoospermy index (TZI), Deformity index(SDI), Multiple anomalies index (MIA), Number of spermatozoa with Excess residual cytoplasm (ERC) |
* this might change according the chosen Morphology classification
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Veterinary catalog online |
Download SCA® Veterinary catalog  Download SCA® Toxicology catalog |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |