SCA® Acrosome reaction is a module that automatically provides the percentage of acrosome intact and acrosome reacted spermatozoa in semen sample of mouse and rat.
| This analysis module is also available for Human and Veterinary. |
Acrosome reaction is one of the most important sperm functional tests.
This test measures in the first instance the percentage intact acrosomes of washed/diluted sperm samples. The next step is that we use a biological substitute to induce the acrosome reaction such as Ca++ ionophore.
More information:
SCA® Acrosome reaction main features
SCA® Acrosome reaction main features
Analysis is performed under fluorescence microscopy, in samples stained with FluoAcro kit, that allows the differentiation between acrosome intact sperm (bright green) and acrosome reacted sperm (blue).
Spermatozoa are recognized and classified automatically.
Additional information
Additional information
| Vet Edition |
Tox Edition |
Research Edition |
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| Creating new analysis configurations |  |
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| Modification of the optics, microscope scales and image capture options | |
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| Modification of the particle area cut off parameter for cell detection | |
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| Motorised stage | |
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| Species available: Human | – | – | |
| Species available: All (human, and all animals, including rat and mouse) | – | – | |
| Species available: All animals with oval sperm head | |
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| Species available: rat and mouse | – | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Specific digital Basler camera with 780×580 | |
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| Specific digital Basler camera with 1280×1024 | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Total sperm counted | |
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| Percentage of the counted sperm with acrosome intact and acrosome reacted | |
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| Captured images and distribution charts | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| SCA® dataShare | |
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| SCA® Stage Controller | |
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| SCA® Capture | |
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| SCA® Viewer | |
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| SCA® Editor | |
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| The assessment is under fluorescence microscopy at 40x (Plan Fluor): The specimen is illuminated with light of a specific wavelength, which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths, i.e., of a different color than the absorbed light . |
| Acrosome reaction of spermatozoa is evaluated using FluoAcro kit. |
| Possibility to add or eliminate spermatozoa and/or captures fields |
| Calculation of basic parametres: Number and percentage of spermatozoa with acrosome intact and acrosome reacted. |
| Visualization of the analysis mask superimposed to the original image. |
| Image capture takes 1 second per field |
| Allows capture up to 30 fields of the sample |
| Counting of spermatozoa with acrosome intact and acrosome reacted by color detection. |
| Number and percentage of spermatozoa with acrosome intact and acrosome reacted. |
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Veterinary catalog online |
Download SCA® Veterinary catalog  Download SCA® Toxicology catalog |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |