SCA® Motility and concentration is a module for the automatic analysis of the sperm motility and concentration (sperm count) in a human semen sample.
| This analysis module is also available for Veterinary and Toxicology. |
Progressive sperm motility is extensively known to be related to pregnancy rates.
Motility must be assessed as soon as possible after liquefaction of the sample, preferably at 30 minutes, but in any case within one hour after ejaculation, as WHO recommends, to decrease the effects of dehydratation, Ph or changes in temperature.
More information:
SCA® Motility and concentration last news
SCA® Motility and concentration last news
Sperm Functional Tests
Automatic results of Hyperactivation and Mucus penetration.
Criteria selection
Spermatozoa paths can be visualized by several criteria (WHO5, WHO4…).
Histogram of velocity
New histogram with sperm velocity (VCL – Curvilinear Velocity).
Drift parameter
Option that enables to eliminate the drift movement in the sample.
SCA® Motility and concentration main features
SCA® Motility and concentration main features
SCA® Motility and concentration detects the spermatozoa automatically.
Samples are analysed under phase contrast (positive phase contrast).
Accurate count and motility analysis are carried simultaneously.
Motile sperm is analysed by following the sperm trajectory and the velocity. The results, comprising all kinematic parameters for CASA, are then presented within seconds.
SCA® is a powerful tool for Andrology, as CASA is the only objective method to analyse the total progressive, motile and hyperactive spermatozoa in a sample, hence giving the indication of the sperm fertilizing potential.
The ultimate SCA® version incorporates the innovation of an intelligent filter, is capable to detect the tail of the spermatozoa, thus permitting the correct analysis of samples containing debris.
Additional information
Additional information
| Human Edition |
Research Edition |
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| Creating new analysis configurations |  |
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| Modification of the fps images capture velocity | |
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| Modification of the optics, microscope scales and image capture options | |
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| Choosing analysis chamber (Leja®, Makler®, etc) | |
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| Motorised stage Prior optiscan III | |
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| Particle area selection (sperm head area) | |
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| VCL or VAP, STR, LIN, VAP, Connectivity, Sort, Analysis restrictions | |
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| Species: Human | |
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| Species: All (human, and all animals, including rat and mouse) | – | |
| Species: All animals with oval sperm head | – | |
| Species: rat and mouse | – | |
| Human Edition |
Research Edition |
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| Specific digital Basler camera with 780×580 resolution and 25 fps capture velocity | |
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| Specific digital Basler camera with 1280×1024 resolution and 25 fps capture velocity | |
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| Specific digital Basler camera with 780×580 resolution and up to 75 fps capture velocity | |
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| Specific digital Basler camera with 1280×1024 resolution and up to 200 fps capture velocity | |
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| Human Edition |
Research Edition |
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| Total sperm counted | |
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| WHO 5: Total and percentage of the counted sperm that are Static, Non progressive and progressive | |
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| Concentration in M/ml and in the sample volume | |
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| Concentration in M/ml and in the sample volume of Static, Non-progressive and Progressive | |
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| Total analysed and Percentage of the Rapid, Medium, Slow and Static | |
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| Concentration in M/ml and in the sample volume the Rapid, Medium, Slow and Static | |
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| WHO 4: Total analysed and percentage of Type A, B, C and D | |
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| WHO 4: Concentration in M/ml and in the sample volume of the Type A, B, C and D. | |
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| Head area average in square µm | |
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| Head area in Static, Slow, Medium and Rapid spermatozoa analysed | |
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| Average of Round Cells in M/ml | |
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| Total Round Cells concentration in M/ml | |
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| Total analysed and percentage of Circular tracks | |
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| Average of VCL, VSL, VAP, LIN, STR, WOB in analysed spermatozoa | |
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| Average of VCL, VSL, VAP, LIN, STR, WOB on Slow, Medium and Rapid Spermatozoa analysed | |
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| Total and percentage of Hyperactive spermatozoa analysed | |
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| Concentration of Hyperactive spermatozoa M/ml and M/Sample | |
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| Average values of ALH, and BCF in analysed spermatozoa | |
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| ALH and BCF values in Slow and Rapid progressive sperm | |
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| Captured images and distribution charts | |
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| Captured Videos | |
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| Human Edition |
Research Edition |
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| SCA® dataShare | |
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| SCA® Stage Controller |
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| SCA® Capture | |
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| SCA® Viewer | |
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| SCA® Editor | |
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| Motility and concentration are analyzed in Phase contrast microscopy (an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image These phase shifts, although invisible, become visible when shown as brightness variations allowing the clear visualization of the semen sample). |
| Or Fluorescence (The specimen is illuminated with light of a specific wavelength which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light) |
| Images are captured with high frame rate digital camera Basler (25 , 50, 75 or up to 200 fps) |
| Automatic detection of non homogeneous fields, allowing a consistent analysis |
| There is a sort function that permits to create groups of sperm population, useful in any sperm research. |
| Modifiable parameters: Particle area for sperm detection, Velocity Classification, VAP, Frame rate, Connectivity, Circularity, Parameter restriction, for example as elongation (to improve the cell detection) |
| Other modifiable parameters: color mask of the path. |
| Allows capture up to 30 fields of the sample |
| Capture time is 1 second per field |
| Intelligent filter that automatically eliminate captured debris in human semen samples |
| Compatible with several disposable analysis chambers like Leja® or Makler® counting chamber |
| Counting |
| Kinematic parameters: VCL, curvilinear velocity (m/s). Time-averaged velocity of a sperm head along its actual curvilinear path, as perceived in two dimensions in the microscope. A measure of cell vigour. VSL, straight-line (rectilinear) velocity (µm/s). Time-averaged velocity of a sperm head along the straight line between its first detected position and its last. VAP, average path velocity (µm/s). Time-averaged velocity of a sperm head along its average path. This path is computed by smoothing the curvilinear trajectory according to algorithms in the SCA Motility module. ALH, amplitude of lateral head displacement (µm). Magnitude of lateral displacement of a sperm head about its average path. It can be expressed as a maximum or an average of such displacements. LIN, linearity. The linearity of a curvilinear path, VSL/VCL. WOB, wobble. A measure of oscillation of the actual path about the average path, VAP/VCL. STR, straightness. Linearity of the average path, VSL/VAP. BCF, beat-cross frequency (Hz). The average rate at which the curvilinear path crosses the average path. |
| Sample (ejaculate or straws) Concentration in M/ml. |
| Total analysed spermatozoa , Percentage in the sample, concentration in M/ml and in the sample volume of the spermatozoa classified by: |
| Velocity: Rapid, Medium, slow and Static |
| WHO 4 classification: Type A (rapid and progressive), B (medium progressive), C(non progressive) and D (static or immotile) |
| WHO 5 classification: Progressive (Type A+B), Motile (Type A+B+C), Static (Type D) |
| Hyperactive |
| Average of the kinematic parameters presented in the sample |
| Total of the head Area / velocity type |
| Total of Round cells / velocity type |
| Total and percentage of circular tracks |
| ALH and BCF in Medium and Rapid Progressive Sperm |
Find further explanations of the CASA Terminology in WHO Laboratory Manual for the Examination and processing of human semen.
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Human catalog online |
Download SCA® Human catalog  |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |