SCA® Acrosome reaction sperm analysis with CASA

SCA® Acrosome reaction is a module that automatically provides the percentage of acrosome intact and acrosome reacted spermatozoa in a human semen sample.

This analysis module is also
available for Veterinary and Toxicology.

Acrosome reaction is one of the most important sperm functional tests.

This test measures in the first instance the percentage intact acrosomes of washed/diluted sperm samples. The next step is that we use a biological substitute to induce the acrosome reaction such as Ca++ ionophore.

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SCA® Acrosome reaction main features

Analysis is performed under fluorescence microscopy, in samples stained with FluoAcro kit, that allows the differentiation between acrosome intact sperm (bright green) and acrosome reacted sperm (blue).

Spermatozoa are recognized and classified automatically.

Additional information

SCA® Acrosome reaction in SCA® Editions

Set up options

	Human Edition	Research Edition
Creating new analysis configurations
Modification of the optics, microscope scales and image capture options
Modification of the particle area cut off parameter for cell detection
Motorised stage
Species available: Human
Species available: All (human, and all animals, including rat and mouse)	–
Species available: All animals with oval sperm head	–
Species available: rat and mouse	–

Digital camera options

	Human Edition	Research Edition
Specific digital Basler camera with 780×580
Specific digital Basler camera with 1280×1024

Results provided

	Human Edition	Research Edition
Total sperm counted
Percentage of the counted sperm with acrosome intact and acrosome reacted
Captured images and distribution charts

Compatible modules

	Human Edition	Research Edition
SCA® dataShare
SCA® Stage Controller
SCA® Capture
SCA® Viewer
SCA® Editor

Technical specifications

Specifications

The assessment is under fluorescence microscopy at 40x (Plan Fluor): The specimen is illuminated with light of a specific wavelength, which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths, i.e., of a different color than the absorbed light .

Acrosome reaction of spermatozoa is evaluated using FluoAcro kit.

Possibility to add or eliminate spermatozoa and/or captures fields

Calculation of basic parametres: Number and percentage of spermatozoa with acrosome intact and acrosome reacted.

Visualization of the analysis mask superimposed to the original image.

Image capture takes 1 second per field

Allows capture up to 30 fields of the sample