Sperm Cryopreservation: Animal diluents and automatic analysis
The first observations concerning low-temperature preservation of spermatozoa date from 1776, when the Italian physiologist Spallanzani noted that spermatozoa cooled in snow became inactive but could be revived on warming, although, successful cryopreservation protocols date only from the 1950s. In 1949, the single most important development came with the discovery by Polge, Smith and Parkes [...]