SCA® DNA Fragmentation is a module for the automatic counting of DNA fragmented and non fragmented spermatozoa, in samples of mouse and rat treated with kits based in the Sperm Chromatin Dispersion (SCD).
| This analysis module is also available for Human and Veterinary. |
DNA fragmentation represents a distinguish mark of apoptosis, and is usually associated with structural changes in cellular morphology.
More information:
SCA® DNA Fragmentation last news
SCA® DNA Fragmentation last news
Analysis improved
DNA Fragmentation analysis and sperm detection improved.
Interactive threshold
Interactive threshold modification for sperm classification.
Export images
Export .bmp or .jpg images including or excluding the analysis.
Individual parameters
Export to Excel or save into the database the individual sperm parameters.
SCA® DNA Fragmentation main features
SCA® DNA Fragmentation main features
Spermatozoa are recognized and classified automatically into fragmented or non fragmented spermatozoa, following the established analysis criteria.
Additional information
Additional information
| Vet Edition |
Tox Edition |
Research Edition |
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| Creating new analysis configurations |  |
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| Modification of the optics, microscope scales and image capture options | |
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| Modification of the particle area cut off parameter for cell detection | |
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| Motorised stage | |
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| Species available: Human | – | – | |
| Species available: All (human, and all animals, including rat and mouse) | – | – | |
| Species available: All animals with oval sperm head (boar, bull, stallion, etc) | |
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| Species available: rat and mouse | – | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Specific digital Basler camera with 780×580 | |
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| Specific digital Basler camera with 1280×1024 | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| Total sperm counted | |
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| Percentage of the counted sperm that are Fragmented and non Fragmented | |
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| Total analysed and Percentage of cells with halo: small, big, medium, degraded and without halo | |
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| Captured images and distribution charts | |
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| Vet Edition |
Tox Edition |
Research Edition |
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| SCA® dataShare | |
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| SCA® Stage Controller | |
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| SCA® Capture | |
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| SCA® Viewer | |
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| SCA® Editor | |
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| DNA fragmentation can be analysed under brightfield microscopy, transmitting light to view a specimen that is stained. |
| Or Fluorescence (The specimen is illuminated with light of a specific wavelength which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light) |
| Disposables: GoldCyto DNA for animal or any preparation based in the Sperm Chromatin Dispersion (SCD). |
| SCA permits to capture and analyse up to 500 spermatozoa per sample |
| Counting of fragmented and non fragmented spermatozoa by halo area or halo-core ratio automatic assessment. |
| Basic results: Number and percentage of fragmented and non fragmented spermatozoa |
| Advanced parameters: Number and percentage of spermatozoa having big, medium, small hallo, degraded and without |
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Veterinary catalog online |
Download SCA® Veterinary catalog  Download SCA® Toxicology catalog |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |