SCA® Vitality is a module that automatically provides the percentage of live and dead spermatozoa in a human semen sample.
| This analysis module is also available for Veterinary and Toxicology. |
Sperm Vitality is estimated by assessing the membrane integrity of the cells, and is fundamental for samples having less than 40% progressive motile spermatozoa. The percentage of the viable cells normally exceeds that of motile cells.
Vitality assessment can be performed with Vitality slides SCA (in brightfield) or with FluoVit kit (under fluorescence).
More information:
SCA® Vitality last news
SCA® Vitality last news
Brightfield analysis
New vitality analysis in brightfield, in samples stained with Vitality slides SCA.
Vitality slides SCA
Ready for the new Vitality slides SCA to assess the Vitality in brightfield.
Interactive threshold
Interactive threshold modification for sperm classification.
Analysis improved
Vitality analysis and sperm detection improved.
SCA® Vitality main features
SCA® Vitality main features
Analysis is performed in brightfield, in samples stained with Vitality slides SCA and can be also performed under fluorescence microscopy with FluoVit kit.
Spermatozoa are recognized and classified automatically (in brightfield, alive sperm in white and dead sperm in red).
Fluorescence analysis allows the differentiation between alive sperm (in blue), and the dead sperm (in red).
Additional information
Additional information
| Human Edition |
Research Edition |
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| Creating new analysis configurations |  |
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| Modification of the optics, microscope scales and image capture options | |
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| Modification of the particle area cut off parameter for cell detection | |
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| Motorised stage | |
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| Species available: Human | |
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| Species available: All (human, and all animals, including rat and mouse) | – | |
| Species available: All animals with oval sperm head | – | |
| Species available: rat and mouse | – | |
| Human Edition |
Research Edition |
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| Specific digital Basler camera with 780×580 | |
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| Specific digital Basler camera with 1280×1024 | |
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| Human Edition |
Research Edition |
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| Total sperm counted | |
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| Percentage of the counted sperm that are alive and dead | |
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| Captured images and distribution charts | |
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| Human Edition |
Research Edition |
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| SCA® dataShare | |
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| SCA® Stage Controller | |
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| SCA® Capture | |
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| SCA® Viewer | |
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| SCA® Editor | |
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| The assessment is under fluorescence microscopy at 20x: The specimen is illuminated with light of a specific wavelength, which is absorbed by the fluorochromes, causing them to emit light of longer wavelengths, i.e., of a different color than the absorbed light . |
| Vitality of spermatozoa is evaluated indirectly though the integrity of the cellular membrane using vital fluorescence staining FluoVit®. |
| Vitality analysis can be also assessed in brightfield at 20x using the Vitality slides SCA. |
| Possibility to add or eliminate spermatozoa and/or captures fields |
| Calculation of basic parametres: Number and percentage of live and dead spermatozoa. |
| Visualization of the analysis mask superimposed to the original image. |
| Image capture takes 1 second per field |
| Allows capture up to 30 fields of the sample |
| Counting of live and dead spermatozoa by color detection |
| Number and percentage of live and dead spermatozoa |
Image gallery
Image gallery
Video
Video
Links
Links
| View SCA® Human catalog online |
Download SCA® Human catalog  |
| Other documents and support: Articles, Manuals, Tutorials, Protocols, mySCA, Technical request |